Third K. H. Kuo Summer School of Electron Microscopy and Crystallography

International Workshop of 3D Molecular Imaging

 

by Cryo-Electron Microscopy

 

Institute of Biophysics, Chinese Academy of Sciences, Beijing, China

August 8 - 12, 2010

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Topics:

1. Single particle analysis
2. Electron tomography
3. Automatic data collection for  cryo-EM
4. Sample preparation for  cryo-EM
5. Electron microscopy density based modeling  
6. Practices on sample preparation, data collection and imaging processing

Recommended reading materials:

Cryo-electron microscopy of viruses. Adrian M, Dubochet J, Lepault J, McDowall AW. Nature. 1984; 308 (5954):32-6.

 

Cryo-electron microscopy of vitrified specimens. Dubochet J, Adrian M, Chang JJ, Homo JC, Lepault J, McDowall AW, Schultz P. Q RevBiophys. 1988; 21(2):129-228. Review.

 

Retrospective on the early development of cryoelectron microscopy of macromolecules and a prospective on opportunities for the future. Taylor KA, Glaeser RM., J Struct Biol. 2008; 163(3):214-23.

 

The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules. Henderson R. Q Rev Biophys. 1995; 28(2):171-93.

 

Determination of icosahedral virus structures by electron cryomicroscopy at subnanometer resolution. Zhou ZH, Chiu W. AdvProtein Chem. 2003; 64:93-124.

 

Electron crystallography of proteins in membranes. Fujiyoshi Y, Unwin N. Curr Opin Struct Biol. 2008; 18(5):587-92. Epub 2008 Sep 11.

 

Three-dimensional reconstruction of single particles in electron microscopy image processing. Radermacher M, Ruiz T. Methods Mol Biol. 2006; 319:427-61.

 

Three-dimensional analysis of single particles by electron microscopy: sample preparation and data acquisition. Ruiz T, Radermacher M. Methods Mol Biol. 2006; 319:403-25.

 

Structure determination of macromolecular assemblies by single-particle analysis of cryo-electron micrographs. Orlova EV, Saibil HR. Curr Opin Struct Biol. 2004; 14(5):584-90. Review.

 

Suggested Books:

"Electron Crystallography of Biological Macromolecules"

Robert Glaeser, Ken Downing, David DeRosier, Wah Chiu and Joachim Frank. Oxford University Press (2007).
 

"Electron Tomography Methods for Three-Dimensional Visualization of Structrures in the Cell" (2nd Edition)

Joachim Frank (2006)

 

"Three-Dimensional electron microscopy of macromolecular assemblies Visualization of biological Molecules in Their Native State"

Joachim Frank (2006)

 

"High-Resolution Electron Microscopy" (3rd Edition)

John C. H. Spence (2003)

 

"Transmission Electron Microscopy Physics of Image Formation" (5th Edition)

Ludwig Reimer, Helmut Kohl (2008)